Product Description:

Cupid-p53-D peptide:

• Cargo: Residues 356 to 393 of human p53 isoform a

• Domain type: C-Terminal DNA binding modulation region

• 38 Amino acids

GKEPGGSRAHSSHLKSKKGQSTS

RHKKLMFKTEGPDSD

Product Characteristics:

• Molecular weight (daltons): 11733

• Isoelectric point (pI): 9.91

• Extinction Coefficient (A280 reduced) : 6990

• Solubility : >200 micromolar

• Purity: 90%

• Cell-Permeation : Passes

• 1 Unit = 10 nanomoles = 0.117 mg

Cupid-p53-D Data:

Cell permeating Cupid p53 peptide DA. Purified Cupid-p53-D peptide was subjected to SDS-PAGE alongside a prestained molecular marker ladder. The gel was then stained for protein with a commercial coomassie-based stain.

M = Weight markers shown in kD

S  = Cupid-p53-D peptide sample

Cell permeating Cupid

p53 Peptide D

B. B. Cupid Control peptide labelled with fluorescein was subjected to SDS-PAGE and observed with a blue light transilluminator.

M = Weight markers shown in Kd, S = Labelled Cupid Control peptide sample.

Comment: The labelled peptide appears to undergo partial association of the monomers into dimers and trimers etc. resulting in a ‘laddering’ effect under the SDS-PAGE conditions.

C. Labelled peptide was incubated with living cells at 10 micromolar for 60 minutes before exchanging the media and washing the cells. Cells were imaged using a fluorescence microscope with filter sets for Fluorescein (Upper Panel) or phase contrast (Lower Panel). Fluorescent images of treated cells are taken at a setting where the background (autofluorescence) of the untreated cells is at the threshold of detection. We observe the peptide fluorescence distributed diffusely throughout all cells.