Cupid-p53-A

Cupid-p53-A

Cupid-p53-A

Product Description:


Cupid-p53-A peptide:

• Cargo: Residues 2 to 42 of human p53 isoform a

• Domain type: Transactivation domain (MDM2 binding)

• 41 Amino acids


EEPQSDPSVEPPLSQETFSDLWKLL

PENNVLSPLPSQAMDD


Product Characteristics:


• Molecular weight (daltons): 12191

• Isoelectric point (pI): 6.3

• Extinction Coefficient (A280 reduced) : 12490

• Solubility : >200 micromolar

• Purity: 80%

• Cell-Permeation : Passes

• 1 Unit = 10 nanomoles = 0.122 mg


Cupid-p53-A Peptide Data

Cupid-p53-A Peptide Data:


Cell permeating Cupid p53 peptide AA. Purified Cupid-p53-A peptide was subjected to SDS-PAGE alongside a prestained molecular marker ladder. The gel was then stained for protein with a commercial coomassie-based stain.


M = Weight markers shown in kD

S  = Cupid-p53-A peptide sample.

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Cell permeating Cupid p53 peptide A

Cell permeating Cupid p53 peptide A


B. Cupid-p53-A peptide labelled with fluorescein was subjected to SDS-PAGE and observed with a blue light transilluminator.


M = Weight markers shown in Kd

S = Labelled Cupid-p53-A peptide sample.


C. Labelled peptide was incubated with living cells at 10 micromolar for 60 minutes before exchanging the media and washing the cells. Cells were imaged using a fluorescence microscope with filter sets for Fluorescein (Upper Panel) or phase contrast (Lower Panel). Fluorescent images of treated cells are taken at a setting where the background (autofluorescence) of the untreated cells is at the threshold of detection. We observe the peptide fluorescence distributed diffusely throughout all cells.

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