Proof of Principle 2

Cell permeable peptide blockade of

PTEN interaction with Drebrin

 

The interaction of PTEN with Drebrin was observed in vivo by co-expression of GFP-PTEN with mCherry-Drebin in PC12 cells. Interaction was analyzed by measuring fluorescence resonance energy transfer (FRET) using multiphoton fluorescence-lifetime imaging microscopy (FLIM) and demonstrated these proteins bound together in a complex and this complex regulates the phosphorylation state of Drebrin

 

Addition of a cell permeable peptide containing the D-Loop peptide (part of the PTEN protein) resulted in separating these proteins by competing for PTEN D-loop interactions.

 

The Cupid-Linked D-Loop peptide is now available (see Cupid-PTEN-B)

Proof of Concept 1 B
Proof of Concept A1
Proof of Concept c1

Phosphorylation of the actin binding protein Drebrin at S647 is regulated by neuronal activity and PTEN.

 

Kreis P, Hendricusdottir R, Kay L, Papageorgiou IE, van Diepen M, Mack T, Ryves J, Harwood A, Leslie NR, Kann O, Parsons M, Eickholt BJ. PLoS One. 2013 Aug 5;8(8):e71957. doi: 10.1371/journal.pone.0071957.

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