Cell permeable peptide blockade of
PTEN interaction with Drebrin
The interaction of PTEN with Drebrin was observed in vivo by co-expression of GFP-PTEN with mCherry-Drebin in PC12 cells. Interaction was analyzed by measuring fluorescence resonance energy transfer (FRET) using multiphoton fluorescence-lifetime imaging microscopy (FLIM) and demonstrated these proteins bound together in a complex and this complex regulates the phosphorylation state of Drebrin
Addition of a cell permeable peptide containing the D-Loop peptide (part of the PTEN protein) resulted in separating these proteins by competing for PTEN D-loop interactions.
The Cupid-Linked D-Loop peptide is now available (see Cupid-PTEN-B)
Kreis P, Hendricusdottir R, Kay L, Papageorgiou IE, van Diepen M, Mack T, Ryves J, Harwood A, Leslie NR, Kann O, Parsons M, Eickholt BJ. PLoS One. 2013 Aug 5;8(8):e71957. doi: 10.1371/journal.pone.0071957.