Cupid-MDM2-C


Cell permeating Cupid MDM2 peptide C

Cupid-MDM2-C

Cupid-MDM2-C

Product Description:

 

Cupid-MDM2-C peptide:

• Cargo: Residues 428 to 479 of human MDM2

• Domain type: zfC3HC4 Motif, Zinc finger, C3HC4 type (RING finger)

• 52 Amino acids

 

SSLPLNAIEPCVICQGRPKNGCIVHGKTGHLM

ACFTCAKKLKKRNKPCPVCR

 

Product Characteristics:

 

• Molecular weight (daltons): 13261

• Isoelectric point (pI): 9.68

• Extinction Coefficient (A280 reduced) : 6990

• Solubility : >200 micromolar

• Purity: 90%

• Cell-Permeation : Passes

• 1 Unit = 10 nanomoles = 0.133 mg

Cupid-MDM2-C Peptide Data

Cupid-MDM2-C Peptide Data:

 

Cell permeating Cupid MDM2 peptide CA. Purified Cupid-MDM2-C peptide was subjected to SDS-PAGE alongside a prestained molecular marker ladder. The gel was then stained for protein with a commercial coomassie-based stain.

 

M = Weight markers shown in kD

 

S = Cupid MDM2-C Peptide sample

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Cell permeating Cupid

MDM2 peptide C

 

B. Cupid-MDM2-C peptide labelled with fluorescein was subjected to SDS-PAGE and observed with a blue light transilluminator.

 

M = Weight markers shown in Kd

 

S = Labelled Cupid-MDM2-C Peptide sample.

 

C. Labelled peptide was incubated with living cells at 10 micromolar for 60 minutes before exchanging the media and washing the cells. Cells were imaged using a fluorescence microscope with filter sets for Fluorescein (Upper Panel) or phase contrast (Lower Panel). Fluorescent images of treated cells are taken at a setting where the background (autofluorescence) of the untreated cells is at the threshold of detection. We observe the peptide fluorescence distributed diffusely throughout all cells.

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