Cupid-MDM2-B


Cell permeating Cupid MDM2 peptide B

Cupid-MDM2-B

Cupid-MDM2-B

Product Description:

 

Cupid-MDM2-B peptide:

• Cargo: Residues 299 to 328 of human MDM2

• Domain type: zf-RAN-BP Motif, Zn-finger in Ran binding protein

• 30 Amino acids

 

LADYWKCTSCNEMNPPLPSHCNRC

WALREN

 

Product Characteristics:

 

• Molecular weight (daltons): 11163

• Isoelectric point (pI): 8.86

• Extinction Coefficient (A280 reduced) : 19840

• Solubility : >200 micromolar

• Purity: 90%

• Cell-Permeation : Passes

• 1 Unit = 10 nanomoles = 0.112 mg

Cupid-MDM2-B Peptide Data

Cupid-MDM2-B Peptide Data:

 

Cell permeating Cupid MDM2 peptide BA. Purified Cupid-MDM2-B peptide was subjected to SDS-PAGE alongside a prestained molecular marker ladder. The gel was then stained for protein with a commercial coomassie-based stain.

 

M = Weight markers shown in kD

 

S = Cupid MDM2-B Peptide sample

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Cell permeating Cupid

MDM2 peptide B

 

B. Cupid-MDM2-B peptide labelled with fluorescein was subjected to SDS-PAGE and observed with a blue light transilluminator.

 

M = Weight markers shown in Kd

 

S = Labelled Cupid-MDM2-B Peptide sample.

 

C. Labelled peptide was incubated with living cells at 10 micromolar for 60 minutes before exchanging the media and washing the cells. Cells were imaged using a fluorescence microscope with filter sets for Fluorescein (Upper Panel) or phase contrast (Lower Panel). Fluorescent images of treated cells are taken at a setting where the background (autofluorescence) of the untreated cells is at the threshold of detection. We observe the peptide fluorescence distributed diffusely throughout all cells.

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