Cupid-PKI


Cell permeating Cupid PKI peptide

Cell Permeating Cupid PKI Peptide

 

B. Cupid-PKI peptide labelled with fluorescein was subjected to SDS-PAGE and observed with a blue light transilluminator.

 

M = Weight markers shown in Kd

 

S = Labelled Cupid-PKI peptide sample.

 

C. Labelled peptide was incubated with living cells at 10 micromolar for 60 minutes before exchanging the media and washing the cells. Cells were imaged using a fluorescence microscope with filter sets for Fluorescein (Upper Panel) or phase contrast (Lower Panel). Fluorescent images of treated cells are taken at a setting where the background (autofluorescence) of the untreated cells is at the threshold of detection. We observe the peptide fluorescence distributed diffusely throughout all cells.

Cupid-PKI

Cupid-PKI

Product Description

 

Cupid-PKI peptide:

• Cargo: Residues 6 to 25 of Human cAMP-dependent protein kinase inhibitor alpha

• Domain type: Pseudo-substrate inhibitor of PKA cat subunit.

• 20 Amino acids

 

TTYADFIASGRTGRRNAIHD

 

 

Product Characteristics:

 

• Molecular weight (daltons): 9833

• Isoelectric point (pI): 9.62

• Extinction Coefficient (A280 reduced) : 8480

• Solubility : >200 micromolar

• Purity: 90%

• Cell-Permeation : Passes

• 1 Unit = 10 nanomoles = 0.098 mg

Cupid-PKI Peptide Data

Cupid-PKI Peptide Data:

 

Cell permeating Cupid PKI peptideA. Purified Cupid-PKI peptide was subjected to SDS-PAGE alongside a prestained molecular marker ladder. The gel was then stained for protein with a commercial coomassie-based stain.

 

M = Weight markers shown in kD

 

S = Cupid PKI peptide sample

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