• Cargo: Residues 316 to 355 of human p53 isoform a
• Domain type: oligomerisation domain
• 40 Amino acids
• Molecular weight (daltons): 12404
• Isoelectric point (pI): 9.24
• Extinction Coefficient (A280 reduced) : 8480
• Solubility : >200 micromolar
• Purity: 90%
• Cell-Permeation : Passes
• 1 Unit = 10 nanomoles = 0.124 mg
Cell permeating Cupid p53 peptide CA. Purified Cupid-p53-C peptide was subjected to SDS-PAGE alongside a prestained molecular marker ladder. The gel was then stained for protein with a commercial coomassie-based stain.
M = Weight markers shown in kD
S = Cupid-p53-C peptide sample
Cell permeating Cupid
p53 peptide C
B. Cupid-p53-C peptide labelled with fluorescein was subjected to SDS-PAGE and observed with a blue light transilluminator.
M = Weight markers shown in Kd, S = Labelled Cupid-p53-C peptide sample.
Comment: The labelled peptide appears to undergo partial association of the monomers into dimers and trimers etc. resulting in a ‘laddering’ effect under the SDS-PAGE conditions.
C. Labelled peptide was incubated with living cells at 10 micromolar for 60 minutes before exchanging the media and washing the cells. Cells were imaged using a fluorescence microscope with filter sets for Fluorescein (Upper Panel) or phase contrast (Lower Panel). Fluorescent images of treated cells are taken at a setting where the background (autofluorescence) of the untreated cells is at the threshold of detection. We observe the peptide fluorescence distributed diffusely throughout all cells.