• Cargo: Residues 2 to 42 of human p53 isoform a
• Domain type: Transactivation domain (MDM2 binding)
• 41 Amino acids
• Molecular weight (daltons): 12191
• Isoelectric point (pI): 6.3
• Extinction Coefficient (A280 reduced) : 12490
• Solubility : >200 micromolar
• Purity: 80%
• Cell-Permeation : Passes
• 1 Unit = 10 nanomoles = 0.122 mg
Cupid-p53-A Peptide Data:
Cell permeating Cupid p53 peptide AA. Purified Cupid-p53-A peptide was subjected to SDS-PAGE alongside a prestained molecular marker ladder. The gel was then stained for protein with a commercial coomassie-based stain.
M = Weight markers shown in kD
S = Cupid-p53-A peptide sample.
Cell permeating Cupid p53 peptide A
B. Cupid-p53-A peptide labelled with fluorescein was subjected to SDS-PAGE and observed with a blue light transilluminator.
M = Weight markers shown in Kd
S = Labelled Cupid-p53-A peptide sample.
C. Labelled peptide was incubated with living cells at 10 micromolar for 60 minutes before exchanging the media and washing the cells. Cells were imaged using a fluorescence microscope with filter sets for Fluorescein (Upper Panel) or phase contrast (Lower Panel). Fluorescent images of treated cells are taken at a setting where the background (autofluorescence) of the untreated cells is at the threshold of detection. We observe the peptide fluorescence distributed diffusely throughout all cells.