Cupid Control Peptide:
The Cupid Control Peptide (Product: Cupid-Control) is the "drill head" peptide without any cargo attached. We have successfully permeated human fibroblasts, kidney cells, chicken neuronal cells and Amoebae with Cupid Control Peptide labelled with fluorescein.
The Cupid Control Peptide serves as an 'experimental control' for Cupid Products to satisfy the researcher that any effects of Cupid Products is due to the cargo attached and not due to other phenomena e.g. an effect of cell permeation itself.
The Cupid Control peptide is fused to the N-terminus of the various cargos we offer and functions to render them 'Cell Permeable'. All Cupid Products have passed the test for Cell Permeability.
The Cupid Control peptide is a large peptide housing "Cupid", our proprietary Cell-Permeating peptide sequence. It also contains stretches of other amino acid residues that assist in the production, processing, purification, solubilisation and cell permeation of Cupid Products.
The feature that might be of most help to a researcher is a stretch of Histidine residues. This can serve to detect the peptide in SDS-PAGE gels / Western blots with a suitable anti-His antibody.
This feature might also be used to pull out proteins or other factors after these have bound to the Cupid Product within cells, although this function has not been tested for this purpose by us. We should be glad to hear of any results you achieve in this regard so we might pass the information on to other users.
We have a limited amount of trial Cupid Control Peptide labelled with Fluorescein which we would be happy to send to researchers who would like to try Cupid Technology under their own cell culture conditions.
All we ask in return is for you to share the results with us so we can provide the information to others which would help them make an informed choice about using our products.