Cell Permeating Cupid PKI Peptide
B. Cupid Control peptide labelled with fluorescein was subjected to SDS-PAGE and observed with a blue light transilluminator.
M = Weight markers shown in Kd,
S = Labelled Cupid Control peptide sample.
C. Labelled peptide was incubated with living cells at 10 micromolar for 60 minutes before exchanging the media and washing the cells. Cells were imaged using a fluorescence microscope with filter sets for Fluorescein (Upper Panel) or phase contrast (Lower Panel). Fluorescent images of treated cells are taken at a setting where the background (autofluorescence) of the untreated cells is at the threshold of detection. We observe the peptide fluorescence distributed diffusely throughout all cells.
Comment: Whilst the Control Peptide has not been observed to have biological effects in doses up to 20 micro molar, Cell-Permeating peptides may be expected to influence certain cell processes through their protein nature. For example those which involve protein recycling.
Cupid Control Peptide
Cupid Control Peptide:
• Cargo: NONE
• Molecular weight (daltons): 7628
• Isoelectric point (pI): 9.43
• Extinction Coefficient (A280 reduced) : 6990
• Solubility : >200 micromolar
• Purity: 88%
• Cell-Permeation : Passes
• 1 Unit = 10 nanomoles = 0.076 mg
Cupid Control Peptide Data:
A. Purified Cupid Control peptide was subjected to SDS-PAGE alongside a prestained molecular marker ladder. The gel was then stained for protein with a commercial coomassie-based stain.
M = Weight markers shown in kD
S = Cupid Control peptide sample