Cupid-Nemo-C


Cupid Nemo C Cell Permeation

Cupid Nemo C Cell Permeation:

 

B. Cupid-Nemo-C peptide labelled with fluorescein was subjected to SDS-PAGE and observed with a blue light transilluminator.

 

M = Weight markers shown in Kd, S = Labelled Cupid-Nemo-C Peptide sample.

 

C. Labelled peptide was incubated with living cells at 10 micromolar for 60 minutes before exchanging the media and washing the cells. Cells were imaged using a fluorescence microscope with filter sets for Fluorescein (Upper Panel) or phase contrast (Lower Panel). Fluorescent images of treated cells are taken at a setting where the background (autofluorescence) of the untreated cells is at the threshold of detection. We observe the peptide fluorescence distributed diffusely throughout all cells.

Cupid-Nemo-C

Cupid-Nemo-C

Cupid Nemo C Peptide:

 

• Cargo: Residues 242 to 350 of Human NF-kappa-B essential modulator isoform a (NEMO)

• Domain type: Ubiquitin binding site

• 109 Amino acids

 

DNHIKSSVVGSERKRGMQLEDLKQQLQQAEEA

LVAKQEVIDKLKEEAEQHKIVMETVPVLKAQAD

IYKADFQAERQAREKLAEKKELLQEQLEQLQRE

YSKLKASCQES

 

Product Characteristics:

 

• Molecular weight (daltons): 20245

• Isoelectric point (pI): 8.25

• Extinction Coefficient (A280 reduced) : 9970

• Solubility : >200 micromolar

• Purity: 89%

• Cell-Permeation : Passes

• 1 Unit = 10 nanomoles = 0.202 mg

Cupid-Nemo-A Peptide Data

Cupid-Nemo-C Peptide Data:

 

A. Purified Cupid-Nemo-C peptide was subjected to SDS-PAGE alongside a prestained molecular marker ladder. The gel was then stained for protein with a commercial coomassie-based stain.

 

M = Weight markers shown in kD

 

S = Cupid Nemo-C Peptide sample

 

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