Cupid-Nemo-B


Cupid Nemo B Cell Permeation

Cupid Nemo B Cell Permeation:

 

B. Cupid-Nemo-B peptide labelled with fluorescein was subjected to SDS-PAGE and observed with a blue light transilluminator.

 

M = Weight markers shown in Kd, S = Labelled Cupid-Nemo-B Peptide sample.

 

C. Labelled peptide was incubated with living cells at 10 micromolar for 60 minutes before exchanging the media and washing the cells. Cells were imaged using a fluorescence microscope with filter sets for Fluorescein (Upper Panel) or phase contrast (Lower Panel). Fluorescent images of treated cells are taken at a setting where the background (autofluorescence) of the untreated cells is at the threshold of detection. We observe the peptide fluorescence distributed diffusely throughout all cells.

Cupid-Nemo-B

Cupid-Nemo-B

Product Description:

 

Cupid Nemo B peptide:

• Cargo: Residues 100 to 196 of Human NF-kappa-B essential modulator isoform a (NEMO)

• Domain type: First Coil-Coil

• 97 Amino acids

 

ARKLVERLGLEKLDLKRQKEQALREVEHLKRCQ

QQMAEDKASVKAQVTSLLGELQESQSRLEAAT

KECQALEGRARAASEQARQLESEREALQQQHS

 

Product Characteristics:

 

• Molecular weight (daltons): 18676

• Isoelectric point (pI): 9.13

• Extinction Coefficient (A280 reduced) : 6990

• Solubility : >200 micromolar

• Purity: 85%

• Cell-Permeation : Passes

• 1 Unit = 10 nanomoles = 0.187 mg

Cupid-Nemo-B Peptide Dat

Cupid-Nemo-B Peptide Data:

 

A. Purified Cupid-Nemo-B peptide was subjected to SDS-PAGE alongside a prestained molecular marker ladder. The gel was then stained for protein with a commercial coomassie-based stain.

 

M = Weight markers shown in kD

 

S = Cupid Nemo-B Peptide sample

 

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