Transcription Factor Permeation:
B. Cupid-Oct4 protein labelled with fluorescein was subjected to SDS-PAGE and observed with a blue light transilluminator.
M = Weight markers shown in Kd, S = Labelled Cupid-Oct4 protein sample.
C. Labelled protein was incubated with living cells at 5 micromolar for 60 minutes before exchanging the media and washing the cells. Cells were imaged using a fluorescence microscope with filter sets for Fluorescein (Upper Panel) or phase contrast (Lower Panel). Fluorescent images of treated cells are taken at a setting where the background (autofluorescence) of the untreated cells is at the threshold of detection. We observe the Cupid-Oct4 protein fluorescence distributed diffusely throughout all cells.
Cupid-Oct4 Transcription Factor
• Cargo: Residues 2 to 360 of human Oct4
• Domain type: : Full Human Protein.
• 359 Amino acids
PPYEFCGG MAYCGPQVGVG LVPQGG LETSQPE
• Molecular weight (daltons): 46036
• Isoelectric point (pI): 7.22
• Extinction Coefficient (A280 reduced) : 43430
• Solubility : >200 micromolar
• Purity: 85%
• Cell-Permeation : Passes
• 1 Unit = 10 nanomoles = 0.46 mg
Cupid-Oct4 Protein Data
A. Purified Cupid-Oct4 protein was subjected to SDS-PAGE alongside a prestained molecular marker ladder. The gel was then stained for protein with a commercial coomassie-based stain.
M = Weight markers shown in kD
S = Cupid-Oct4 protein sample