Cupid-cMyc-Transcription-Factor


Cupid-cMyc Transcription Factor Permeation

Cupid-cMyc

Transcription Factor Permeation:

 

B. Cupid-cMyc protein labelled with fluorescein was subjected to SDS-PAGE and observed with a blue light transilluminator.

 

M = Weight markers shown in Kd, S = Labelled Cupid-cMyc protein sample.

 

C. Labelled protein was incubated with living cells at 5 micromolar for 60 minutes before exchanging the media and washing the cells. Cells were imaged using a fluorescence microscope with filter sets for Fluorescein (Upper Panel) or phase contrast (Lower Panel). Fluorescent images of treated cells are taken at a setting where the background (autofluorescence) of the untreated cells is at the threshold of detection. We observe the Cupid-cMyc protein fluorescence distributed diffusely throughout all cells.

Cupid-cMyc Transcription Factor

Cupid-cMyc Transcription Factor

Product Description:

 

Cupid-cMyc Protein:

• Cargo: Residues 2 to 454 of human cMyc

• Domain type: : Full Human Protein.

• 453 Amino acids

 

DFFRWENQQPPATMPLNVSFTNRNYDLDYDS

VQPYFYCDEEEN FYQQQQQSELQPPAPSEDIW

KKFELLPTPPLSPSRRSGLCSPSWAVTPFSLRGD

NDGGGGSFSTADQLEMVTELLGGDMVNQSFIC

DPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLAS

YOAARKDSGSPNPARGHSVCSTSSLYLQDLSAA

ASECIDPSWFPYPLN DSSSPKSCASQDSSAFSPS

SDSLLSSTESSPQGSPEPLVLHEETPPTTSSDSEEE

QEDEEElDWSVEKRQAPGKRSESGSPSAGGHS

KPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPA

AKRVKLDSVRVLRQISNNRKCTSPRSSDTEENVK

RRTHNVLERQRRNELKRSFFALRDQIPELENNEK

APKVVILKKATAYILSVQAEEQKLISEEDLLRKRRE

QLKH KLEQLRNSCA

 

Product Characteristics:

 

• Molecular weight (daltons): 58044

• Isoelectric point (pI): 6.12

• Extinction Coefficient (A280 reduced) : 35870

• Solubility : >200 micromolar

• Purity: 85%

• Cell-Permeation : Passes

• 1 Unit = 10 nanomoles = 0.58 mg

Cupid-cMyc Protein Data

Cupid-cMyc Protein Data

 

A. Purified Cupid-cMyc protein was subjected to SDS-PAGE alongside a prestained molecular marker ladder. The gel was then stained for protein with a commercial coomassie-based stain.

 

M = Weight markers shown in kD

 

S = Cupid-cMyc protein sample

 

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